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KMID : 0615720120290010103
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.103 ~ p.113
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Effect of Suppressing the Activation of Macrophage Migration Inhibitory Factor by Sambucus williamsii HANCE Extract & Pharmacopuncture Solution on Type 2 Collagen-induced Arthritis
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Lee Dong-Gun
Kim Eun-Jung
Lee Eun-Sol
Wang Kai-Hsia
Jo Hyun-Seok
Lee Seung-Deok
Kim Kap-Sung
Kim Kyung-Ho
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Abstract
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Objectives: The purpose of this study is to evaluate effect of suppressing the expression of cyclo-oxygenase-type-2 (COX-2) as a consequence of inhibition macrophage migration inhibitory factor (MIF) activation by Sambucus williamsii Hance (SWH) pharmacopunctureon rheumatoid arthritis (RA).
Methods: In vitro test, synoviocytes extracted from type Ⅱ collagen-induced arthritis (CIA) mouse’s knee joint were cultivated After that, each well of synoviocytes was mixed with the extract of SWH at the dosage of 0.4 mg/㎖, 0.6 mg/㎖, 0.8 mg/㎖, and 1.0 mg/㎖ respectively, and cultivated for 24 hours after the addition. Reverse transcriptase - polymerase chain reaction (RT-PCR) is used to investigate the expression of MIF, Tumor necrosis factor (TNF)-α, COX-2 mRNA. In vivo test, thirty DBA female mice were used, and each ten mice were allocated into three group; normal group, CIA-elicitated group, and group treated with SWH pharmacopuncture on it’s the point of ST35 after CIA elicitation. It is investigated that change of mice foot thickness, histologic change of sliced synovial joint of mouse, and extent change of MIF, TNF-α, COX-2 in synovial membrane.
Results: In vitro test, the expressions of cytokine(MIF, TNF-α, COX-2) mRNAs related to RA were dose-dependent decreased. In the SWH pharmacopuncture group, foot thickness and histologic change of sliced synovial joint were decreased comparing with CIA-elicitated group’s change. In the SWH pharmacopuncture group, the suppression of MIF, TNF-α, COX-2 in synovial membrane was clearly shown comparing with CIA-elicitated group’s change.
Conslusions: It might be suggested that SWH pharmacopuncture mitigate tissue damage originated from rheumatoid arthritis by suppressing the expression of COX-2 as a consequence of inhibition MIF activation.
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KEYWORD
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Sambucus williamsii HANCE, pharmacopuncture, rheumatoid arthritis, collagen induced Arthritis
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